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PCR protocols : a guide to methods and applications /

by Innis, Michael A.

DNA-Directed DNA Polymerase.

Gene Amplification -- methods.

Genetic Engineering -- methods.

DNA-Directed RNA Polymerases.

0123721806 (alk. paper)

0123721814 (pbk. : alk. paper)

xviii, 482 p. : ill. ; 24 cm.

Optimization of PCRs / Michael A. Innis and David H. Gelfand -- Amplification of genomic DNA / Randall K. Saiki -- Amplification of RNA / Ernest S. Kawasaki -- RACE : rapid amplification of cDNA ends / Michael A. Frohman -- Degenerate primers for DNA amplification / Teresa Compton -- cDNA cloning using degenerate primers / Cheng Chi Lee and C. Thomas Caskey -- PCR with 7-Deaza-2'-deoxyguanosine triphosphate / Michael A. Innis -- Competitive PCR for quantitation of mRNA / Gary Gilliland, Steven Perrin, and H. Franklin Bunn -- Quantitative PCR / Alice M. Wang and David F. Mark -- Production of single-stranded DNA by asymmetric PCR / Peter C. McCabe -- Cloning with PCR / Stephen J. Scharf -- Oligonucleotide ligation assay / Ulf Landegren, Robert Kaiser, and Leroy Hood.

Nonisotopically labeled probes and primers / Corey Levenson and Chu-an Chang -- Incorporation of biotinylated dUTP / Y.-M. Dennis Lo, Wajahat Z. Mehal, and Kenneth A. Fleming -- Nonisotopic detection of PCR products / Rhea Helmuth -- Thermostable DNA polymerases / David H. Gelfand and Thomas J. White -- Procedures to minimize PCR-product carry-over / Shirley Kwok -- Sample preparation from blood, cells, and other fluids / Ernest S. Kawasaki -- Sample preparation from paraffin-embedded tissues / Deann K. Wright and M. Michele Manos -- Amplifying ancient DNA / Svante Pääbo -- In vitro transcription of PCR templates / Michael J. Holland and Michael A. Innis -- Recombinant PCR / Russell Higuchi -- DNase I footprinting / Barbara Krummel -- Sequencing with Taq DNA polymerase / Mary Ann D. Brow.

Direct sequencing with the aid of phage promoters / Steve S. Sommer ... [et al.] -- Identifying DNA polymorphisms by denaturing gradient gel electrophoresis / Val C. Sheffield, David R. Cox, and Richard M. Meyers -- Amplification of flanking sequences by inverse PCR / Howard Ochman ... [et al.] -- Detection of homologous recombinants / Michael A. Frohman and Gail R. Martin -- RNA processing : Apo-B / Lyn M. Powell -- A transcription-based amplification system / T.R. Gingeras ... [et al.] -- Screening of [lambda] gt11 libraries / Kenneth D. Friedman ... [et al.] -- HLA DNA typing / Henry A. Erlich and Teodorica L. Bugawan -- Multiplex PCR for the diagnosis of Duchenne muscular dystrophy / Jeffrey S. Chamberlain ... [et al.] -- Isolation of DNA from fungal mycelia and single spores / Steven B. Lee and John W. Taylor -- Genetic prediction of hemophilia A / Scott C. Kogan and Jane Gitschier.

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